Short Description: An Open-Access, Peer-Reviewed, Google Scholar indexed, Cabells WHITE-LISTED journal, publishing scholarly articles in Biology, Medicine, Agriculture & Allied Sciences.
E-ISSN: 2378-654X
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Publisher: HATASO - SynergyGlobal
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10.18639/RABM.2025.9800042
Microbiology and Immunology
Feb 15, 2025
This study investigated the enzymatic activities of proteases, DNases, and hemolysins across five clinically relevant bacterial strains: Among the various bacterial species in the contaminated foods and drinks, the most common are – E. coli, S. aureus, P. aeruginosa, S. pyogenes, and B. cereus. Protease activity was analyzed using casein solution to evaluate the relative optical density, while DNase activity was determined on DNase agar plates, observing the clear zones on the agar media, and hemolysin activity was checked on blood agar plates. The contention here is that the mean diameter of clear zones was determined for die strains and we prevailed to analyze the differences in die enzymatic activities. Our findings also highlighted differences in the bacterial strains studied with regard to a number of factors including protease, DNase and haemolytic activity: P. aeruginosa displayed the highest protease activity while S. aureus and B. cereus were observed to have high DNase activity while S. pyogenes displayed the highest haemolytic activity. The protease and DNase parameters were further analyzed by means of correlation coefficients, and the results revealed a linear correlation between the two at +0.983; however, the hemolysin activity was not directly related to either protease or DNase. Altogether the current observations offer perspectives regarding the numerous strategies that bacteria have adapted in infecting host tissues and producing damage, as well as the significance of studying enzymatic processes in an effort to develop specific pharmacological treatments.
10.18639/RABM.2024.9800041
Microbiology and Immunology
Dec 27, 2024
In recent times, especially during the antibiotic resistance era, it is crucial to find active and sustainable alternatives that work against microbes. In this study, seven plants Minuartia nifensis McNeill, Sideritis sipylea Boiss, Asperula daphneola O.Schwarz, Teucrium montanum L, Stachys cretica L. subsp, Smyrnaea rech. Fil and Euphorbia erythrodon Boiss/Heldr growing in Turkey were collected and newly registered. Alcoholic extracts were prepared to investigate their antimicrobial and antioxidant activity. These plants are widespread in the mountains of Izmir City (Turkey). Three assessments were performed in this project: agar well diffusion method, a standard antimicrobial (antibacterial and anti-fungal) activity test, followed by MIC test, which determines the lowest concentration and lowest quantity that affects microbes after adding resazurin indicator, and finally, antioxidant activity by ELISA microplate reader. The findings showed activity against S. aureus and E. colį. Antifungal activity against C. albįcans and C. glabrata was the highest activity shown by most extracts. Antioxidant activity was also confirmed by quantification of radical scavenging activity via DPPH assay.
10.18639/RABM.2024.9800040
Short Communication
May 07, 2024
The purpose of this work is to summarize the biological methods available for assessing DNA damage in humans at present. There are several methods for determining single-strand DNA breaks, alkali-labile sites, and crosslinks, including comet assays, micronucleus assays, cytogenetics (which include sister chromatid exchange and chromosomal aberration assays), DNA repair assays, oxidative DNA damage assays (measuring oxidized bases such as 8-oxo-7, 8-dihydro-2'-deoxyguanosine), and oxidized bases. There are many factors to consider when determining the best method, including how to achieve the study's objectives, what type of DNA damage to measure, and what resources are available. Combining different techniques may also contribute to a more comprehensive understanding of DNA damage and its effects on human health. By standardizing assays and advancing technology, we will be able to determine DNA damage in humans more accurately.
