Short Description: An Open-Access, Peer-Reviewed, Google Scholar indexed, Cabells WHITE-LISTED journal, publishing scholarly articles in Biology, Medicine, Agriculture & Allied Sciences.
E-ISSN: 2378-654X
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Publisher: HATASO - SynergyGlobal
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10.18639/RABM.2024.9800041
Microbiology and Immunology
Dec 27, 2024
In recent times, especially during the antibiotic resistance era, it is crucial to find active and sustainable alternatives that work against microbes. In this study, seven plants Minuartia nifensis McNeill, Sideritis sipylea Boiss, Asperula daphneola O.Schwarz, Teucrium montanum L, Stachys cretica L. subsp, Smyrnaea rech. Fil and Euphorbia erythrodon Boiss/Heldr growing in Turkey were collected and newly registered. Alcoholic extracts were prepared to investigate their antimicrobial and antioxidant activity. These plants are widespread in the mountains of Izmir City (Turkey). Three assessments were performed in this project: agar well diffusion method, a standard antimicrobial (antibacterial and anti-fungal) activity test, followed by MIC test, which determines the lowest concentration and lowest quantity that affects microbes after adding resazurin indicator, and finally, antioxidant activity by ELISA microplate reader. The findings showed activity against S. aureus and E. colį. Antifungal activity against C. albįcans and C. glabrata was the highest activity shown by most extracts. Antioxidant activity was also confirmed by quantification of radical scavenging activity via DPPH assay.
10.18639/RABM.2024.9800040
Short Communication
May 07, 2024
The purpose of this work is to summarize the biological methods available for assessing DNA damage in humans at present. There are several methods for determining single-strand DNA breaks, alkali-labile sites, and crosslinks, including comet assays, micronucleus assays, cytogenetics (which include sister chromatid exchange and chromosomal aberration assays), DNA repair assays, oxidative DNA damage assays (measuring oxidized bases such as 8-oxo-7, 8-dihydro-2'-deoxyguanosine), and oxidized bases. There are many factors to consider when determining the best method, including how to achieve the study's objectives, what type of DNA damage to measure, and what resources are available. Combining different techniques may also contribute to a more comprehensive understanding of DNA damage and its effects on human health. By standardizing assays and advancing technology, we will be able to determine DNA damage in humans more accurately.
10.18639/RABM.2024.9800039
Toxicology and Pharmacology
Jan 25, 2024
Diclofenac is a non-steroidal anti-inflammatory drug commonly used in the treatment of pain and inflammatory conditions in the tropics. Its easy accessibility without prescription has made it a commonly abused medication. The abuse has recently become worrisome as young adults in Nigeria have been observed to use diclofenac and alcohol for non-medical purposes concomitantly. This study was undertaken to study the nephrotoxic effect associated with the combined use of alcohol and diclofenac using an animal model and the protective effect of Vitis vinifera (Grape) seed and Carica papaya (pawpaw) seed. Adult albino rats were divided randomly into groups of six rats each, and each group was treated for 90 days with 5% or 45% alcohol with 2mg/kg diclofenac or 10mg/kg diclofenac, and some rats were treated with grape seed and/or pawpaw seed concomitantly. Diclofenac and 45% alcohol combination induced nephrotoxicity in rats and oxidative stress characterized by elevated serum urea and creatinine, increased malondialdehyde and decrease in reduced glutathione, catalase and superoxide dismutase in kidney homogenate, as well as moderate interstitial congestion and hemorrhage in rat kidney histology section stained with Haematoxylin and Eosin, which was significantly ameliorated by the grape seed and pawpaw seed. There was a 1.32% increase in relative kidney weight in comparison with control rats. The severity of the nephrotoxicity observed with the diclofenac-alcohol combination makes it a very unsafe practice. There is a need to explore the nephroprotective benefits of Vitis vinifera seed and Carica papaya seed for maximum benefit to man.
