Measuring Body Size in Small Marine Fishes: A Comparison of Three Non-intrusive Methods
Studies of non-intrusive techniques are important in fisheries biology, because research methods may inadvertently cause
damage to the study organisms. In addition, current effects of human–environment interactions coupled with future trends in
global climate change likely will lead to increased monitoring of fish population dynamics. The aim of this study is to analyze
the effectiveness of three simple non-intrusive techniques to accurately obtain body length measurements of anemonefish
and other small fishes. Frequently used catch and re-capture methods are stressful to fishes, and can alter their behaviors
upon release, thus negatively impacting field ecological studies. Alternate methods to non-intrusive sizing of reef fishes
are needed, and these methods should be compared to determine the most effective and efficient means of collecting the
targeted data. Three non-intrusive techniques were employed to obtain accurate fork length (FL) measurements of the twoband anemonefish, Amphiprion bicinctus. Comparison of these methods revealed that fish lengths from visual estimates
by self-contained under water breathing apparatus (SCUBA) divers did not differ significantly from those estimated
using both video-mirror and Tps-mirror techniques (ANOVA, F(2,60) 5 1.572; p 5 0.22). Under laboratory conditions, fish
sizes from manual measurements also did not differ significantly from those obtained using either mirror method (ANOVA,
F(2,81) 5 0.489; p 5 0.61), demonstrating that the mirror techniques accurately assess fish size under both laboratory and
field conditions. These methods were not effective in identifying or tracking individual fish among years in the field, due to
high rates of fish mobility and turnover. However, they were useful in determining short-term anemonefish migration among
sea anemone hosts.
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