To estimate the long-term effect of carbon dioxide (CO2) emission on cereal yield in Sudan, we employed an autoregressive distributed lagged (ARDL) bound test for cointegration analysis. The ARDL results reveal evidence of cointegration between the dependent variable (cereals yield) and two independent variables (CO2 emission) and agricultural GDP. The estimation results of the error correction model indicate that change in CO2 has a positive and significant impact on the cereal yield in the long and short terms, as 1% increase in CO2 leads to a cereal yield increase by 3% in the short term and by 0.7% in the long term. This result adds two important findings to the existing literature: First, the positive impact of CO2 on cereal yield in Sudan supports previous research findings in other countries of warm and arid climates. Second, the effect of CO2 on cereal yield differs from short to long term, as our finding indicates that CO2 has a greater positive effect in the short term compared to that in the long term, implying that the effect of CO2 on cereal yields is not linear, as commonly perceived, but it decreases as time duration extends to longer periods. This may be due to the CO2 effect on global warming that emanates from cumulative CO2 concentration, which leaves a disproportionate impact on crops over time.