This study was carried out on Allium sativum and Persea americana seeds’ extracts, to compare their alkaloid content and to evaluate the antihyperglycemic potentials solely and in combination, as well as the effect of the combination on sucrose digestion in normal Wistar rats. Aqueous extract (AE) and hydroethanolic extract (HEE) of both plants were prepared. Alkaloid content was determined. Antihyperglycemic activity was evaluated by oral glucose tolerance test and sucrose digestion for 120 min. Normoglycemic albino Wistar male rats of 150-220 g were used. Rats were distributed in fi ve groups of six each: positive control group (water), four test groups (400 mg/kg b.w. HEE and AE of A.s and P.a), and reference group (4 mg/kg b.w., glibenclamide). All the groups received 2 g/kg b.w. of glucose solution. Similar design was followed to test formulation (1:1, AE of A.s 200 and HEE of P.a 200 extracts) and for sucrose (2 g/kg bw) digestion, using acarbose as reference drug. Glycemia was followed up for 30, 60, 90, and 120 min after administration of substrates. HEE Pa revealed eight folds of alkaloid content higher than A.s extracts. Both the individual extracts (AE A.s: 1563; HEE P.a: 5270) and the formulation had lower area under the curve (AUC) values (combination: 2825 mg min/dL) compared to positive control (4015 mg min/dL; p 0.05). However, AE As400 effi cacy was comparable to glibenclamide (1163 mg min/dL; p 0.05). The HEE Pa, AE As400, and the formulation (AEAs200 1 HEEAs200) on sucrose digestion revealed higher AUC values compared to positive control. We concluded that AE As200 combined with HEE Pa200 ameliorates the glucose lowering potential of the HEE of P. americana but increases the sucrose digestion rate.
