Section : Research Article

Published Date : Jan 25,2013

Lede

Choosing potential dissolution medium to study the influence of water-insoluble substances on aggregation of platelets within preclinical studies under conditions in vitro

Abstract

The authors have studied the influence of dissolution media of potential antiaggregant in order to further carry out preclinical research of their impact on functional activity of platelets. There has been defined different impact of ethanol, dimethylsufoxide (DMSO), dimethylformamide (DMFA), and dioxane on the hemostasis system. It has been verified that dioxane, DMFA, and DMSO exert evident influence on adenosine diphosphate (ADP)- and collagen-induced aggregation of platelets, which is determined by turbidimetric method. The findings enable using ethanol as a solvent in maximum 50% exposure (vol/vol) in order to study the impact on aggregation of platelets of water-insoluble substances by this method.