Fish sperm motility nowadays is considered as the best biomarker for the quality of fish spermatozoa, and sperm motion parameters from more than 300 fish species have been reported in more than 1500 scientific articles covering a wide range of topics, from molecular biology to ecology. The present study was conducted to evaluate two lab prepared activation media (A, B) and control (distilled water) on the milt quality in male and fertilization rate in female American channel catfish (Ictalurus punctatus). Brooders were developed from locally bred catfish seed and selected on the basis of maturity atAquaculture and Fisheries Program, National Agriculture Research Center (NARC), Islamabad Pakistan. The experiment used a Complete Randomized Design (CRD) with three replicates for each of the three treatments: media A, B, and Control (distilled water). As millions of spermsare present in per micro liter of sperm therefore, for more effective use of sperm or milt, different types of activation or dilution media were used. After collection of milt itwas diluted with the above corresponding activation media in ratio of 1:29 followed by in-vitro analysis for various parameters including sperm mobility, sperm mobility duration and sperm viability. Fertilization rates was checked by using same diluted milt aliquots and the results revealed that the components were significantly different (p<0.05) in the prescribed conducts. The sperm/egg aliquot treated with medium "B" had the highest mobility/motility and fertilization rates (81.66% ± 2.88 to 85.33% ± 2.08), followed by media "A" (73.33% ± 2.80to 71.66% ± 2.08), and control (68.33% ± 2.8 to 67.33% ± 2.08). Sperm viabilityand concentration in A, B, and C, or thecontrol, were likewise substantially different (p<0.05) from one another. The media “B” had the greatest values (61.66% ± 2.53 to 2.50% ± 0.14), followed by "A" (57.33% ± 2.51 to 2.37% ± 0.76), and "Control" (48.33% ± 1.15 to 1.93% ± 0.45). The length of the sperm mobilityduration varied considerably (p<0.05) across all treatments. It was measured in seconds and was 320sec ± 34.64 for the control, 570sec ± 43.58 for media "B" alone, and 471sec ± 10.14 for media "A" with means and standard deviations.
