Short Description: "Biology and Medicine" (also referred to as BLM or BAM) is a peer-reviewed, ONLINE HYBRID OPEN ACCESS journal, efficiently publishing original and relevant articles in thrust areas of research in Biological and Medical Sciences.
E-ISSN: 0974-8369
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Publisher: Walsh Medical Media
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City: London
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Country: United Kingdom
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Email: [email protected]
Research Article
Nov 11, 2022
Background: Chronic Myeloid Leukemia (CML) is one of malignant hematologic disorders arises from hematopoietic stem cells. BCR-ABL transcript levels on the international scale at 3 and 6 months are defined as indicators of the early efficacy of first line TKI treatment. Aim To investigate the impact of Early Molecular Response (EMR; BCR-ABL ≤ 10% on the International scale at 3 or 6 months) on outcome of the newly diagnosed CML in chronic phase treated with Nilotinib. Patients and Methods: The study was enrolled from 2018 to 2020 at Nasser Institute for Research and Treatment. This is a prospective cohort study done on 94 newly diagnosed CML cases in Chronic Phase. Results: A statistically significant difference was detected between patients not achieved EMR with peripheral blasts ≥ 5%, when compared to others achieved EMR with peripheral blasts <5% (P<0.001). 75% of patients not achieved EMR were ≥ 55 years age at diagnosis; and 90% of patients achieved EMR were<55 years of age at diagnosis with (P<0.001). 25% of cases not achieved EMR were compliant, while other cases achieved EMR were compliant with (P<0.001).Overall survival remained higher in patients who achieved EMR (N=90) compared to patients who did not achieve EMR (N=4) (P=0.0001). Conclusion: EMR is an important prognostic significance for CML patients received treatment with Nilotinib. Patients suffered with who achieved EMR had significantly better outcome. Achieving MR3.0 should be the aim in patents with CML- CP who have a 3-month BCR-ABL ≤ 10% and 6-month BCR-ABL ≤ 10%.
Research Article
Nov 01, 2022
A cross sectional study was conducted in Worabe town, Southern Ethiopia, to assess milk handling practice, determine physicochemical properties and evaluate the microbial quality of raw cow milk produced in the town. Three kebele were purposely selected based on their dairy production potential one kebele from town two kebele from rural. A total of 120 dairy farms consisting of crossbred dairy cattle were selected. The result of the survey indicates that the majority of the respondents (86.7%) follow milking procedures that include washing hands and utensils before and after milking, while 91.7% of the producers wash the udder and teats before milking. Majority of the respondents (100%) used purchased feeds in the farm and the main source of water was tap water. A total of 30 raw milk samples were collected and laboratory work was conducted to determine microbial load. The results of overall means revealed that 4.54 ± 0.67 cfu/ml total bacteria count, 2.95 ± 0.44 cfu/ml ColiForm count and 2.63 ± 0.46 cfu/ml yeast and mould count. There was a significance variation (p<0.001) for total bacteria count and yeast and mould among the three kebele no significance difference for ColiForm count. The result revealed that overall mean for specific gravity (density), water, fat, protein, solid-non-fat and total solid milk samples were 1.02 ± 0.02, 88.54 ± 1.51, 3.54 ± 0.76, 3.23 ± 0.61, 8.15 ± 3.00 and 11.46 ± 1.51 respectively. Except SNF and TS the physicochemical property of milk from all milk production was under the acceptable level. The microbial quality raw milk produced by the milk shed was poor. Therefore, to ensure safety and quality of milk and health of the public, strict hygienic practice should be followed during milk production and handling.
Case Report
Oct 25, 2022
Rigidity and Multifocal Seizure Syndrome, Lethal Neonatal (RMFSL, OMIM#614498), is caused by mutations of the BRAT1 gene. Our patient had the typical syndromes of RMFSL, and Trio whole exome sequencing (trio-WES) identified a homozygous synonymous variant (BRAT1:C.1395 (exon10) G>C). Given that the pathogenicity of synonymous mutation (p.Thr465Thr) is most likely underestimated, a further transcriptional study of the father showed that C.1395 (exon10) G>C mutation would result in abnormal splicing, which caused the exon 10 skipping and affected protein features. We first confirmed the pathogenicity of the synonymous mutation of BRAT1 using a transcriptional study.
